The Bayview Immunomics Core (BIC) was initially established in 2008 as a flow cytometry core to meet the biomedical research needs of Johns Hopkins Investigators located on the Bayview Campus. We have since expanded to include immunoassays and genomic services and renamed the core the BIC. The BIC is directed by Mark J. Soloski, Ph.D. The flow cytometry service center is managed by Raffaello Cimbro who brings the highest standard of flow cytometry experience to the modern biomedical sciences. The core is uniquely embedded among research groups interested in problems related to immune based human diseases and relevant animal models and promotes an interactive and collaborative approach. We have an extensive research base and considerable expertise in flow based approaches to address neuroscience related research. The flow cytometry instrumentation has the capability of performing simple and complex multiparametric (up to 14 fluorochromes) analysis of cell subpopulations and high speed sorting. We are presently equipped with a special order FACSAria cell sorter (BD Biosciences) with 14-fluorescence parameter capability, a six color FACSCanto (BD Biosciences), and several analysis workstations for off-line data analysis. The FACSAria and FACSCanto are maintained using the highest standards of quality control and are available for analysis to certified and trained users. Cell sorting is performed by flow core staff only. Our staff offers free consultation services.
We also have on site a Meso Scale system capable of sensitive (subnanogram) multiplex based detection of soluble mediators in fuid sources. Meso Scale developed an electrochemiluminescence-based detection system that offers several advantages in comparison to standard ELISAs including high sensitivity, a broad dynamic range, low background and increased reproducibility. This instrument employs standard or custom plates, and assays can be run and read by core or investigator’s lab staff. Access requires an initial consult.
In addition, we offer Genomics Services including purification of RNA from cell/tissue sources and the generation of libraries for next generation deep sequencing. This effort is led by Jinshui Fan Ph.D. who has over a decade of expertise in this area. Access to genomic services requires an initial consult.
The Johns Hopkins Bayview Immunomics Core was formed to support the research initiatives of Johns Hopkins Bayview Faculty and staff. We are committed to providing state-of-the art technology and staff support to address current flow cytometry, cell sorting, immunomics applications and new strategies. Please feel free to contact us for flow cytometry and cell sorting consultation services.